β1 v2e9 antibodies Search Results


β1 integrins  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank β1 integrins
β1 Integrins, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hybridoma culture supernatant  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank hybridoma culture supernatant
Hybridoma Culture Supernatant, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β1 integrin antibodies chicken integrin α5  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank β1 integrin antibodies chicken integrin α5
β1 Integrin Antibodies Chicken Integrin α5, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chick β1 integrin subunit  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank chick β1 integrin subunit
Effect of <t> anti-β1-integrin </t> antibodies on neurite outgrowth of sensory neurons grown for 48 hr in Terasaki dishes on laminin substrata
Chick β1 Integrin Subunit, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibodies jg22  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank monoclonal antibodies jg22
Confocal analysis of neurite outgrowth of embryonic chicken neurons cultivated on rat sciatic nerve sections. Cultures were fixed after 48 hr and stained using an <t>mAb</t> directed against chicken N-CAM, revealed with FITC. Laminin-2 (merosin) in the substratum was decorated with an antiserum revealed with TRITC.A–D and 1–4, Culture on normal nerve section. A, Complete reconstruction, by extended depth of focus, of a growing neuron and underlying substratum, obtained by superimposition of consecutive images in the horizontal plane. The neuritic arborization (green) is confined to two adjacent Schwann cell tubes, defined by laminin-2 immunoreactive basal lamina scaffolds. B–D, Consecutive horizontal optical sections of the same neuron, obtained at 5 μm intervals. The plane of image C lies at midlevel of the Schwann cell tube. On normal nerve substratum, neurites extend in contact with laminin-immunoreactive basal laminae (arrows). Note inC that they do not grow inside Schwann cell tubes.Insets 1–4, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1–4 shown in A. Such optical sections confirm that neurites grow in contact with basal laminae (arrowheads) but do not extend inside Schwann cell tubes, despite the fact that some neurites are found deep inside the tissue section. E–F and 1′–4′, Culture on degenerated nerve section. E, Complete reconstruction as in A of a growing neuron and underlying substratum. The neuritic arborization of the neuron seen on theright is confined to a single Schwann cell tube. Neurites of another neuron (cell body not visible in the micrograph) extend along a second basal lamina scaffold, on the leftof the previous neuron. F–H, Consecutive horizontal optical sections of the same field, obtained at 5 μm intervals. The plane of image G lies at midlevel of the Schwann cell tube. On denervated substrata, neurites also extend in contact with laminin-immunoreactive basal laminae (arrows). However, note in G that they grow profusely inside Schwann cell tubes. Insets 1′–4′, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1′–4′ shown in E. Such optical sections confirm that on denervated nerve sections neurites grow not only in contact with basal laminae (arrowheads) but also extend inside Schwann cell tubes (arrows). Scale bars: A–D, E–H, 25 μm; 1–4 and 1′–4′, 10 μm.
Monoclonal Antibodies Jg22, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chicken n cam  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank chicken n cam
Confocal analysis of neurite outgrowth of embryonic chicken neurons cultivated on rat sciatic nerve sections. Cultures were fixed after 48 hr and stained using an <t>mAb</t> directed against chicken N-CAM, revealed with FITC. Laminin-2 (merosin) in the substratum was decorated with an antiserum revealed with TRITC.A–D and 1–4, Culture on normal nerve section. A, Complete reconstruction, by extended depth of focus, of a growing neuron and underlying substratum, obtained by superimposition of consecutive images in the horizontal plane. The neuritic arborization (green) is confined to two adjacent Schwann cell tubes, defined by laminin-2 immunoreactive basal lamina scaffolds. B–D, Consecutive horizontal optical sections of the same neuron, obtained at 5 μm intervals. The plane of image C lies at midlevel of the Schwann cell tube. On normal nerve substratum, neurites extend in contact with laminin-immunoreactive basal laminae (arrows). Note inC that they do not grow inside Schwann cell tubes.Insets 1–4, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1–4 shown in A. Such optical sections confirm that neurites grow in contact with basal laminae (arrowheads) but do not extend inside Schwann cell tubes, despite the fact that some neurites are found deep inside the tissue section. E–F and 1′–4′, Culture on degenerated nerve section. E, Complete reconstruction as in A of a growing neuron and underlying substratum. The neuritic arborization of the neuron seen on theright is confined to a single Schwann cell tube. Neurites of another neuron (cell body not visible in the micrograph) extend along a second basal lamina scaffold, on the leftof the previous neuron. F–H, Consecutive horizontal optical sections of the same field, obtained at 5 μm intervals. The plane of image G lies at midlevel of the Schwann cell tube. On denervated substrata, neurites also extend in contact with laminin-immunoreactive basal laminae (arrows). However, note in G that they grow profusely inside Schwann cell tubes. Insets 1′–4′, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1′–4′ shown in E. Such optical sections confirm that on denervated nerve sections neurites grow not only in contact with basal laminae (arrowheads) but also extend inside Schwann cell tubes (arrows). Scale bars: A–D, E–H, 25 μm; 1–4 and 1′–4′, 10 μm.
Chicken N Cam, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effect of anti-β1-integrin antibodies on neurite outgrowth of sensory neurons grown for 48 hr in Terasaki dishes on laminin substrata

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Effect of anti-β1-integrin antibodies on neurite outgrowth of sensory neurons grown for 48 hr in Terasaki dishes on laminin substrata

Article Snippet: Antibodies The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Inhibition

Inhibition of neurite outgrowth of E13 chick sensory neurons by two different antibodies, JG22 and V2E9, directed against chick β1-integrin subunit, on each type of nerve substratum. The concentrations used were 25 μg Ig/ml for both antibodies. The blocking ability of each antibody was controlled with the same neuronal preparations grown on laminin-1 or laminin-2, and the corresponding results are shown in Table ​Table1.1. A, Quantification of the number of neurons extending neurites after 2 d of culture. Values represent mean and SEM of the percentage of growing neurons for five different experiments. B, Quantification of neurite length. Values represent the mean and SEM of total neuritic length elaborated per neuron, measured for at least 120 neurons.*p < 0.05. Significance was analyzed using the Student’s t test.

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Inhibition of neurite outgrowth of E13 chick sensory neurons by two different antibodies, JG22 and V2E9, directed against chick β1-integrin subunit, on each type of nerve substratum. The concentrations used were 25 μg Ig/ml for both antibodies. The blocking ability of each antibody was controlled with the same neuronal preparations grown on laminin-1 or laminin-2, and the corresponding results are shown in Table ​Table1.1. A, Quantification of the number of neurons extending neurites after 2 d of culture. Values represent mean and SEM of the percentage of growing neurons for five different experiments. B, Quantification of neurite length. Values represent the mean and SEM of total neuritic length elaborated per neuron, measured for at least 120 neurons.*p < 0.05. Significance was analyzed using the Student’s t test.

Article Snippet: Antibodies The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Inhibition, Blocking Assay

Effects of anti-β1-integrin antibodies (JG22) and of anti-ECM molecule antibodies on the number of primary neurites elaborated per sensory neuron, cultivated on sections of normal and degenerated sciatic nerve

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Effects of anti-β1-integrin antibodies (JG22) and of anti-ECM molecule antibodies on the number of primary neurites elaborated per sensory neuron, cultivated on sections of normal and degenerated sciatic nerve

Article Snippet: Antibodies The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Inhibition

Confocal analysis of neurite outgrowth of embryonic chicken neurons cultivated on rat sciatic nerve sections. Cultures were fixed after 48 hr and stained using an mAb directed against chicken N-CAM, revealed with FITC. Laminin-2 (merosin) in the substratum was decorated with an antiserum revealed with TRITC.A–D and 1–4, Culture on normal nerve section. A, Complete reconstruction, by extended depth of focus, of a growing neuron and underlying substratum, obtained by superimposition of consecutive images in the horizontal plane. The neuritic arborization (green) is confined to two adjacent Schwann cell tubes, defined by laminin-2 immunoreactive basal lamina scaffolds. B–D, Consecutive horizontal optical sections of the same neuron, obtained at 5 μm intervals. The plane of image C lies at midlevel of the Schwann cell tube. On normal nerve substratum, neurites extend in contact with laminin-immunoreactive basal laminae (arrows). Note inC that they do not grow inside Schwann cell tubes.Insets 1–4, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1–4 shown in A. Such optical sections confirm that neurites grow in contact with basal laminae (arrowheads) but do not extend inside Schwann cell tubes, despite the fact that some neurites are found deep inside the tissue section. E–F and 1′–4′, Culture on degenerated nerve section. E, Complete reconstruction as in A of a growing neuron and underlying substratum. The neuritic arborization of the neuron seen on theright is confined to a single Schwann cell tube. Neurites of another neuron (cell body not visible in the micrograph) extend along a second basal lamina scaffold, on the leftof the previous neuron. F–H, Consecutive horizontal optical sections of the same field, obtained at 5 μm intervals. The plane of image G lies at midlevel of the Schwann cell tube. On denervated substrata, neurites also extend in contact with laminin-immunoreactive basal laminae (arrows). However, note in G that they grow profusely inside Schwann cell tubes. Insets 1′–4′, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1′–4′ shown in E. Such optical sections confirm that on denervated nerve sections neurites grow not only in contact with basal laminae (arrowheads) but also extend inside Schwann cell tubes (arrows). Scale bars: A–D, E–H, 25 μm; 1–4 and 1′–4′, 10 μm.

Journal: The Journal of Neuroscience

Article Title: Comparison of Neurite Outgrowth Induced by Intact and Injured Sciatic Nerves: A Confocal and Functional Analysis

doi: 10.1523/JNEUROSCI.18-01-00328.1998

Figure Lengend Snippet: Confocal analysis of neurite outgrowth of embryonic chicken neurons cultivated on rat sciatic nerve sections. Cultures were fixed after 48 hr and stained using an mAb directed against chicken N-CAM, revealed with FITC. Laminin-2 (merosin) in the substratum was decorated with an antiserum revealed with TRITC.A–D and 1–4, Culture on normal nerve section. A, Complete reconstruction, by extended depth of focus, of a growing neuron and underlying substratum, obtained by superimposition of consecutive images in the horizontal plane. The neuritic arborization (green) is confined to two adjacent Schwann cell tubes, defined by laminin-2 immunoreactive basal lamina scaffolds. B–D, Consecutive horizontal optical sections of the same neuron, obtained at 5 μm intervals. The plane of image C lies at midlevel of the Schwann cell tube. On normal nerve substratum, neurites extend in contact with laminin-immunoreactive basal laminae (arrows). Note inC that they do not grow inside Schwann cell tubes.Insets 1–4, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1–4 shown in A. Such optical sections confirm that neurites grow in contact with basal laminae (arrowheads) but do not extend inside Schwann cell tubes, despite the fact that some neurites are found deep inside the tissue section. E–F and 1′–4′, Culture on degenerated nerve section. E, Complete reconstruction as in A of a growing neuron and underlying substratum. The neuritic arborization of the neuron seen on theright is confined to a single Schwann cell tube. Neurites of another neuron (cell body not visible in the micrograph) extend along a second basal lamina scaffold, on the leftof the previous neuron. F–H, Consecutive horizontal optical sections of the same field, obtained at 5 μm intervals. The plane of image G lies at midlevel of the Schwann cell tube. On denervated substrata, neurites also extend in contact with laminin-immunoreactive basal laminae (arrows). However, note in G that they grow profusely inside Schwann cell tubes. Insets 1′–4′, Successive vertical optical sections performed perpendicular to the long axis of Schwann cell tubes, along the lines 1′–4′ shown in E. Such optical sections confirm that on denervated nerve sections neurites grow not only in contact with basal laminae (arrowheads) but also extend inside Schwann cell tubes (arrows). Scale bars: A–D, E–H, 25 μm; 1–4 and 1′–4′, 10 μm.

Article Snippet: The monoclonal antibodies (mAbs) directed against the chick β1-integrin subunit, JG22 (David I. Gottlieb; Greve and Gottlieb, 1982 ), V2E9 (Alan F. Horwitz; Hayashi et al., 1990 ), and against chicken N-CAM, 5e (Urs Rutishauser), were obtained from the Developmental Studies Hybridoma Bank maintained by the Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, and the Department of Biological Sciences, University of Iowa, Iowa City, IA 52242, under contract N01-HD-6-2915 from the National Institute of Child Health and Human Development.

Techniques: Staining